Protocols

Current protocols used by the lab, shared to faciliate open science. Revised: August 5, 2022

• Growing Arabidopsis

  • Note: This protocol has aspects which are unique to the He Lab and our facilities, and may lack details assumed to be understood in our lab.

• Microbotryomycete yeast transformation - Video

  • Note: This protocol also works for other yeasts, such as Aureobasidium pullulans if the Agrobacterium strain and T-DNA is appropriate.

• Leaf sterilization and microbial load counting - Video

• Total phyllosphere microbial load counting - Video

• Arabidopsis mesh pots - Video